Selected Publications

Zhang D. Plant seed-derived human transferrin: expression, characterization, and applications. OA Biotechnology. 2013;2(2):17.

Broz A, Huang N, Unruh G. Plant-based protein biomanufacturing. Genetic Engineering and Biotechnology News. 2013; 33(4).

Zhang D, Lee HF, Pettit S, Zaro JL, Huang N, Shen WC. Characterization of transferrin receptor-mediated endocytosis and cellular iron delivery of recombinant human serum transferrin from rice (Oryza sativa L.). BMC Biotechnology. 2012;12:92.

Steere AN, Bobst CE, Zhang D, Pettit S, Kaltashov IA, Huang N, Mason AB. Biochemical and structural characterization of recombinant human serum transferrin from rice (Oryza sativa L.). J Inorg Biochem. 2012;116:37-44.

Laffan AM, McKenzie R, Forti J, Conklin D, Marcinko R, Shrestha R, Bellantoni M, Greenough WB. Lactoferrin for the prevention of post-antibiotic diarrhoea. J Health Popul Nutr. 2011;29 (6):547-551.

Zhang D, Nandi S, Bryan P, Pettit S, Nguyen D, Santos MA, Huang N. Expression, purification, and characterization of recombinant human transferrin from rice (Oryza sativa L.). Protein Expr Purif. 2010;74 (1):69-79.

Tang L, Cui T, Wu JJ, Liu-Mares W, Huang N, Li J. . A rice-derived recombinant human lactoferrin stimulates fibroblast proliferation, migration, and sustains cell survival. Wound Repair Regen. 2010;18 (1):123-131.

Cerven D, DeGeorge G, Bethell D. 28-Day repeated dose oral toxicity of recombinant human apo-lactoferrin or recombinant human lysozyme in rats. Regul Toxicol Pharmacol. 2008;51 (2):162-167.

Moneret-Vautrin DA, Astier CMN, Franck P, Morisset M, Codreanu F, Roitel O, Taylor SL, Bethell DR. Assessment of the potential cross-allergenicity between hen's egg lysozyme and recombinant human lysozyme. J Allergy Clin Immunol. 2007;119 (1):S109.

Zavaleta N, Figueroa D, Rivera J, Sanchez J, Alfaro S, Lonnerdal B. Efficacy of rice-based oral rehydration solution containing recombinant human lactoferrin and lysozyme in Peruvian children with acute diarrhea. J Pediatr Gastroenterol Nutr. 2007;44 (2):258-264.

Lönnerdal B. Recombinant human milk proteins. In: Rigo J, Ziegler EE, eds. Protein and Energy Requirements in Infancy and Childhood. Karger Publishers; 2006:207-217.

Nandi S, Yalda D, Lu S, Nikolov Z, Misaki R, Fujiyama K, Huang N. Process development and economic evaluation of recombinant human lactoferrin expressed in rice grain. Transgenic Res. 2005;14 (3):237-249.

Hennegan K, Yang D, Nguyen D, Wu L, Goding J, Huang J, Guo F, Huang N, Watkins SC. Improvement of human lysozyme expression in transgenic rice grain by combining wheat (Triticum aestivum) puroindoline b and rice (Oryza sativa) Gt1 promoters and signal peptides. Transgenic Res. 2005;14 (5):583-592.

Hwang YS, Ciceri P, Parsons RL, Moose SP, Schmidt RJ, Huang N. The maize O2 and PBF proteins act additively to promote transcription from storage protein gene promoters in rice endosperm cells. Plant Cell Physiol. 2004;45 (10):1509-1518.

Bethell DR, Huang J. Recombinant human lactoferrin treatment for global health issues: iron deficiency and acute diarrhea. Biometals. 2004;17 (3):337-342.

Yang D, Guo F, Liu B, Huang N, Watkins SC. Expression and localization of human lysozyme in the endosperm of transgenic rice. Planta. 2003;216 (4):597-603.

Huang J, Wu L, Yalda D, Adkins Y, Kelleher SL, Crane M, Lonnerdal B, Rodriguez RL, Huang N. Expression of functional recombinant human lysozyme in transgenic rice cell culture. Transgenic Res. 2002;11 (3):229-239.

Huang J, Nandi S, Wu L, Yalda D, Bartley G, Rodriguez R, Lonnerdal B, Huang N. Expression of natural antimicrobial human lysozyme in rice grains. Molecular Breeding. 2002;10 (1):83-94.

Hwang YS, Yang D, McCullar C, Wu L, Chen L, Pham P, Nandi S, Huang N. Analysis of the rice endosperm-specific globulin promoter in transformed rice cells. Plant Cell Reports. 2002;20 (9):842-847.

Humphrey BD, Huang N, Klasing KC. Rice expressing lactoferrin and lysozyme has antibiotic-like properties when fed to chicks. J Nutrition. 2002;132 (6):1214-1218.

Yang D, Wu L, Hwang YS, Chen L, Huang N. Expression of the REB transcriptional activator in rice grains improves the yield of recombinant proteins whose genes are controlled by a Reb-responsive promoter. Proc Natl Acad Sci U S A. 2001;98 (20):11438-11443.

Huang J, Sutliff TD, Wu L, Nandi S, Benge K, Terashima M, Ralston AH, Drohan W, Huang N, Rodriguez RL. Expression and purification of functional human alpha-1-Antitrypsin from cultured plant cells. Biotechnol Prog. 2001;17 (1):126-133.

Hwang YS, McCullar C, Huang N. Evaluation of expression cassettes in developing rice endosperm using a transient expression assay. Plant Science. 2001;161 (6):1107-1116.

Huang N, Wu L, Nandi S, Bowman E, Huang J, Sutliff T, Rodriguez RL. The tissue-specific activity of a rice beta-glucanase promoter (< i> Gns9) is used to select rice transformants. Plant science. 2001;161 (3):589-595.

von Wettstein D, Mikhaylenko G, Froseth JA, Kannangara CG. Improved barley broiler feed with transgenic malt containing heat-stable (1,3-1,4)-beta-glucanase. Proc Natl Acad Sci U S A. 2000;97 (25):13512-13517.

Terashima M, Murai Y, Kawamura M, Nakanishi S, Stoltz T, Chen L, Drohan W, Rodriguez RL, Katoh S. Production of functional human alpha 1-antitrypsin by plant cell culture. Appl Microbiol Biotechnol. 1999;52 (4):516-523.